<HTML><BODY style="word-wrap: break-word; -khtml-nbsp-mode: space; -khtml-line-break: after-white-space; ">Dear Kepler Users,<DIV><BR class="khtml-block-placeholder"></DIV><DIV>I am a new to the world of workflows and Kepler, so I apologize if my questions are to basic for this list!</DIV><DIV>I am trying to use a web service actor to Blast a sequence in DDBJ. However, for some reason, it is not working properly. Using ddbj or restricting to ddbjpln as the database to be used, the query takes more than 15 minutes to run and at the end I get nothing (e.g. a blank window in a display actor). I do not know what I am doing wrong. I tried to blast the same accession (AJ403010) configuring the web service actor with the BlastDemo.wsdl and it worked fine. Then I tried to Blast the accession directly in DDBJ website, (restricting to ddbjpln) and it worked fine as well. I would appreciate any suggestions.</DIV><DIV><BR class="khtml-block-placeholder"></DIV><DIV>Also, I need to parse this Blast result (I think this can be a tricky step to automatize in Kepler, but I am not sure) . I would like to be able to filter the output based on hits e-values (e.g. -10, -20, etc) and to filter the output for redundancy (e.g not use the same taxon twice). Then I want to retrieve the sequences for that filtered list and put it in Fasta Format. I have not been able to find an easy way to get around this problem in Kepler. Any help would be greatly appreciated!</DIV><DIV><BR class="khtml-block-placeholder"></DIV><DIV>I am using Kepler 1.0 Beta2 for Mac (I have a PowerBook PPC)</DIV><DIV><BR class="khtml-block-placeholder"></DIV><DIV>Felipe</DIV><DIV><DIV> <SPAN class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px 0px; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; text-align: auto; -khtml-text-decorations-in-effect: none; text-indent: 0px; -apple-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><SPAN class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px 0px; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; text-align: auto; -khtml-text-decorations-in-effect: none; 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-khtml-text-decorations-in-effect: none; text-indent: 0px; -apple-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><SPAN class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px 0px; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; text-align: auto; -khtml-text-decorations-in-effect: none; text-indent: 0px; -apple-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><SPAN class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px 0px; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; text-align: auto; -khtml-text-decorations-in-effect: none; text-indent: 0px; -apple-text-size-adjust: auto; 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border-spacing: 0px 0px; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; text-align: auto; -khtml-text-decorations-in-effect: none; text-indent: 0px; -apple-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><P style="margin: 0.0px 0.0px 0.0px 0.0px">:-:-:-:-:-:</P><P style="margin: 0.0px 0.0px 0.0px 0.0px"><FONT face="Helvetica" size="3" style="font: 12.0px Helvetica">Felipe Zapata</FONT></P><P style="margin: 0.0px 0.0px 0.0px 0.0px">UMSL-Biology</P><P style="margin: 0.0px 0.0px 0.0px 0.0px">St. Louis, MO 63121 USA</P><DIV><BR class="khtml-block-placeholder"></DIV></SPAN></SPAN></SPAN></SPAN></SPAN></SPAN></SPAN></SPAN></SPAN></SPAN></SPAN></SPAN></SPAN></SPAN></SPAN></SPAN></DIV><BR></DIV></BODY></HTML>