[kepler-users] WebService-Blast

[Felipe Zapata]

Dear Kepler Users,

I am a new to the world of workflows and Kepler, so I apologize if my  
questions are to basic for this list!
I am trying to use a web service actor to Blast a sequence in DDBJ.   
However, for some reason, it is not working properly.  Using ddbj or  
restricting to ddbjpln as the database to be used, the query takes  
more than 15 minutes to run and at the end I get nothing (e.g. a  
blank window in a display actor).  I do not know what I am doing  
wrong.  I tried to blast the same accession (AJ403010) configuring  
the web service actor with the BlastDemo.wsdl and it worked fine.  
Then I tried to Blast the accession directly in DDBJ website,  
(restricting to ddbjpln) and it worked fine as well.  I would  
appreciate any suggestions.

Also, I need to parse this Blast result (I think this can be a tricky  
step to automatize in Kepler, but I am not sure) . I would like to be  
able to filter the output based on hits e-values (e.g. -10, -20, etc)  
and to filter the output for redundancy (e.g not use the same taxon  
twice). Then I want to retrieve the sequences for that filtered list  
and put it in Fasta Format. I have not been able to find an easy way  
to get around this problem in Kepler. Any help would be greatly  
appreciated!

I am using Kepler 1.0 Beta2 for Mac (I have a PowerBook PPC)

Felipe
:-:-:-:-:-:
Felipe Zapata
UMSL-Biology
St. Louis, MO 63121 USA


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