EML mapping question.

David Blankman dblankman at lternet.edu
Mon Nov 18 12:36:22 PST 2002


Below is a  metadata document from the McMurdo LTER site. In the 
document they have a section called "CITATION".
* CITATIONS: *Spaulding, Sarah A, Diane M. McKnight, Richard L. Smith 
and Richard Dufford. 1994. Phytoplankton population dynamics in 
perennially ice-covered Lake Fryxell, Antarctica. Journal of Plankton 
Research. Vol.16 no.5 pp.527-541.

It appears to be an indication that the data was used in that journal 
article.

Where in the vast scheme of EML would that information be put? My first 
thought was there ought to be something at the dataset level to use. 
When I look at the ResourceGroup, I see pubDate and Series. The 
documentation for series implies that there is a reference to a journal:

<doc:tooltip>Series</doc:tooltip>
            <doc:summary>The series from which the resource 
came.</doc:summary>
            <doc:description>This field describes the series of 
resources that
            include the resource being described. For example, a volume of a
            journal may be part of a series of the journal for a particular
            year.</doc:description>

Perhaps I have been looking at EML too long and can't see the obvious, 
but I couldn't find any obvious place to put this info. I can see that 
one could use the title of the journal article as the title or 
alternative title., but where would the following go:

Journal of Plankton Research. Vol.16 no.5 pp.527-541.



  SUMMER PHYTOPLANKTON DENSITIES 1992-2001

------------------------------------------------------------------------

Acceptance and utilization of LTER data requires that:

    * the Principal Investigator be sent a notice stating reasons for
      acquiring any data and a description of the publication intentions.
    * The Principal Investigator of the data set be sent a copy of the
      report or manuscript prior to submission and be adequately cited
      in any resultant publications.
    * A copy of any resultant publications should be sent to the McMurdo
      data manager at:
      as well as the Principal Investigator. For a list of MCM LTER
      Principal Investigators <http://huey.colorado.edu/LTER/pi.html>.

------------------------------------------------------------------------
SUMMER PHYTOPLANKTON DENSITIES 1992-2001

* FILE NAME: *phytplkt.dat 
<http://huey.colorado.edu/LTER/datasets/lakes/plankton/phytplkt.dat>Data 
File (Comma Delimited Ascii Format).

* PRINCIPAL INVESTIGATOR: *Diane McKnight
*Address:*INSTAAR, 1560 30th Street, Campus Box 450, Boulder CO, 80309-0450
*Phone:*(303)492-4687
*E-Mail:*mcknight at snobear.colorado.edu 
<mailto:mcknight at snobear.colorado.edu>

* OTHERS: *

* KEYWORDS: *lake, limnology, phytoplankton, density, phylum

* ABSTRACT: *Phytoplankton were collected over four austral summers 
(1991-92 through 1994-95) to examine seasonal and annual fluctuation in 
species composition and biovolume in Lakes Fryxell, Hoare, West Lake 
Bonney, and East Lake Bonney. All of these lakes are perennially 
ice-covered lakes located in the Dry Valleys of South Victoria Land, 
Antarctica. The phytoplankton consisted primarily of cryptophyte and 
chlorophyte flagellates, and filamentous cyanobacteria. Some common taxa 
were vertically stratified (Oscillatoria limnetica, Phormidium 
angustissimum, Pyramimonas sp., Oscillatoria sp.), while others showed 
no distinct vertical stratification (Chlamydomonas subcaudata, 
Cryptomonas sp.). The stratification of the phytoplankton reflects the 
gradients of nutrients and light, and the stability of the water column.

* VARIABLES: *limno run, location name, location code, date, depth (m), 
phylum, species, density (cells/mL), file name

* RESEARCH LOCATION: *Lake Fryxell, Lake Bonney and Lake Hoare are all 
located in Taylor Valley, South Victoria Land, Antarctica. Lake Fryxell 
(77 37'S, 163 07'E) is at an elevation of 16 m above sea level. It has 
an area of 7 km2 and a maximum depth of 19 m. The perennial ice cover 
has a thickness of ~4.5 m. Sampling was conducted at a center station, 
where the depth was 18.5 m. Lake Bonney is roughly 6.0 kilometers in 
length and up tp 900 meters wide. It is comprised of a larger east basin 
and a smaller west basin. Samples were gathered from both basins. The 
east lobe has a perennial ice cover thickness of ~3.8 m; the west lobe 
has a perennial ice cover thickness of ~3.7 m. Lake Hoare occupies a 
narrower portion of the valley, is dammed by the Canada Glacier, and 
would drain almost completely without this dam. It is roughly 4.2 
kilometers long and up to 1100 meters wide. There are a number of 
islands which may be related to an old terminal moraine of Canada 
Glacier. The perennial ice cover thickness is ~4.0 m.

* METHODS: *Discrete samples for phytoplankton enumeration were 
collected from the oxygenated portion of the water column (below the 
bottom of the ice to a depth of 10 m) at 0.5 m intervals. Sampling was 
done primarily between the hours of 14:00 and 20:00 (during the austral 
summer, the illuminated period is 24 h/day) by either peristaltic pump 
or Kemmerer bottle. Samples were collected in 1 l bottles and preserved 
immediately with Lugon's solution (American Public Health Association, 
1985). Identification and counts were made with an inverted microscope 
by the method of Utermohl (1958). At least 100 individuals of the most 
numerous algae were counted per sample at 100x magnification. The total 
number of individuals counted was dependent on the number of taxa, but 
ranged between 200 and 500. Counting error ranged between 13 and 26%, 
depending on species. Algal species identifications were made using 
Geitler (1932), Seaburg et al. (1979) and Prescott (1962). Cell volumes 
were estimated for dominant taxa by measuring cell dimensions of 50-100 
individuals and using closest geometric formulas of additional dates and 
depths to determine changes in cell volume over time. For rare taxa, 
volume estimates were made from fewer cell measurements.

Primary productivity was measured using the method of Strickland and 
Parsons (1972). In situ 24 h incubations were made in triplicate 300 ml 
light and duplicate 300 ml dark bottles with Na14CO3 (3 uCi per 300 ml, 
New England Nuclear). Following 24 h incubation, samples were well 
shaken and filtered through Whatman GF/F filters in the dark. The 
filters were placed in scintillation vials and acidified with 1 ml of 5% 
acetic acid in methanol to remove [C14] carbonates. The [C14] fixed by 
biological activity was determined in Aquasol (New England Nuclear) by 
liquid scintillation.

Samples for nitrate and orthophosphate were frozen within several hours 
of collection, later filtered through 0.45 um Nucleopore filters and 
analyzed by air-segmented continuous-flow absorption spectrophotometry 
(Alpkem RFA-300) (Antweiler et al., 1993). Chlorophyll extractions were 
made in 95% ethanol (Jesperson and Christoffersen, 1987) and measured in 
a Turner Designs Model 10 Fluorometer.

* TIMING: *Samples were gathered from the following locations and dates:
*LocationDate(s)*
Lake Fryxell:1/10/92, 11/16/93, 1/7/94, 1/18/94, 11/14/94, 12/4/94, 
1/17/95, 9/14/95, 11/26/95, 12/27/95, 10/26/96, 11/19/96, 1/9/97, 
11/18/97, 12/29/97, 10/28/98, 11/23/98, 12/20/98, 10/30/99, 11/27/99, 
12/23/99, 11/7/00, 12/3/00, 12/30/00, 1/17/01
East Lake Bonney:10/27/93, 11/24/93, 12/21/93, 11/2/94, 12/17/94
West Lake Bonney:10/29/93, 11/26/93, 12/23/93, 11/4/94, 12/19/94
Lake Hoare:11/5/93, 12/1/93, 12/30/93, 1/19/94, 11/10/94, 12/24/94, 
1/20/95, 10/10/95, 11/21/95, 12/23/95, 11/8/96, 12/2/96, 11/14/97, 
12/1/97, 12/26/97, 11/3/98, 12/1/98, 12/26/98, 11/17/99, 12/12/99, 
1/4/00, 11/11/00, 1/2/01

* CITATIONS: *Spaulding, Sarah A, Diane M. McKnight, Richard L. Smith 
and Richard Dufford. 1994. Phytoplankton population dynamics in 
perennially ice-covered Lake Fryxell, Antarctica. Journal of Plankton 
Research. Vol.16 no.5 pp.527-541.

-- 
David E. Blankman
Database Integration Developer
Long Term Ecological Research Network Office
University of New Mexico
801 University, SE #104
Albuquerque, NM 87106
(505) 272-7346 / (505) 272-7080 FAX

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